AP2A1 Polyklonaler Antikörper

AP2A1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30030-1-PBS

Synonyme

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  WB analysis using 30030-1-AP (same clone as 30030-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30030-1-AP (same clone as 30030-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 30030-1-AP (same clone as 30030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30030-1-AP (AP2A1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30030-1-PBS bindet in WB, IHC, Indirect ELISA AP2A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP2A1 fusion protein Ag32232
• Vollständiger Name: adaptor-related protein complex 2, alpha 1 subunit
• Berechnetes Molekulargewicht: 977 aa, 108 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC014214
• Gene symbol: AP2A1
• Gene ID (NCBI): 160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.