AP2B1 Monoklonaler Antikörper

AP2B1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A3B6

Kat-Nr. : 68349-1-PBS

Synonyme

ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1, DKFZp781K0743

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  WB analysis using 68349-1-Ig (same clone as 68349-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68349-1-Ig (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68349-1-Ig (same clone as 68349-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68349-1-Ig (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68349-1-Ig (same clone as 68349-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68349-1-Ig (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68349-1-Ig (same clone as 68349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68349-1-Ig (AP2B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68349-1-Ig (same clone as 68349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68349-1-Ig (AP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68349-1-Ig (same clone as 68349-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AP2B1 antibody (68349-1-Ig, Clone: 1A3B6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68349-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68349-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA AP2B1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AP2B1 fusion protein Ag8264
• Vollständiger Name: adaptor-related protein complex 2, beta 1 subunit
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 100-115 kDa
• GenBank-Zugangsnummer: BC006201
• Gene symbol: AP2B1
• Gene ID (NCBI): 163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.