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AP2S1 Polyklonaler Antikörper

AP2S1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15634-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using AP2S1 Polyclonal antibody (15634-1-AP)

    WB analysis using 15634-1-AP (same clone as 15634-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15634-1-AP (AP2S1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15634-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15634-1-PBS bindet in WB, Indirect ELISA AP2S1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen AP2S1 fusion protein Ag8095
Vollständiger Name adaptor-related protein complex 2, sigma 1 subunit
Berechnetes Molekulargewicht 142 aa, 17 kDa
Beobachtetes Molekulargewicht15-17 kDa
GenBank-ZugangsnummerBC006337
Gene symbol AP2S1
Gene ID (NCBI) 1175
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP2S1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1), and a small adaptin (sigma-type subunit AP2S1). It works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis. Missense mutations of AP2S1 affect Arg15 and lead to familial hypocalciuric hypercalcemia type 3 (FHH3), an extracellular calcium homeostasis disorder affecting the parathyroids, kidneys, and bone (PMID: 23222959).