AP2S1 Rekombinanter Antikörper

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• KD/KO Validated

AP2S1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241369F1

Kat-Nr. : 84174-3-PBS

Synonyme

241369F1, Adaptor protein complex AP-2 subunit sigma, Adaptor-related protein complex 2 subunit sigma, AP 2 complex subunit sigma, AP17

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  WB analysis using 84174-3-RR (same clone as 84174-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84174-3-RR (AP2S1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84174-3-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 84174-3-RR (same clone as 84174-3-PBS)

  WB result of AP2S1 antibody (84174-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP2S1 transfected HEK-293 cells. This data was developed using the same antibody clone with 84174-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84174-3-RR (same clone as 84174-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84174-3-RR (AP2S1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84174-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84174-3-RR (same clone as 84174-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AP2S1 antibody (84174-3-RR, Clone: 241369F1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84174-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84174-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84174-3-RR against Human AP2S1 were performed. The affinity constant is 0.502 nM.

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Geprüfte Anwendungen

Produktinformation

84174-3-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA AP2S1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AP2S1 fusion protein Ag8095
• Vollständiger Name: adaptor-related protein complex 2, sigma 1 subunit
• Berechnetes Molekulargewicht: 142 aa, 17 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC006337
• Gene symbol: AP2S1
• Gene ID (NCBI): 1175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP2S1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes form clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1), and a small adaptin (sigma-type subunit AP2S1). It works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis. Missense mutations of AP2S1 affect Arg15 and lead to familial hypocalciuric hypercalcemia type 3 (FHH3), an extracellular calcium homeostasis disorder affecting the parathyroids, kidneys, and bone (PMID: 23222959).