AP2S1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

AP2S1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241369F1

Kat-Nr. : 84174-3-RR

Synonyme

241369F1, Adaptor protein complex AP-2 subunit sigma, Adaptor-related protein complex 2 subunit sigma, AP 2 complex subunit sigma, AP17

---

• 

  WB analysis using 84174-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84174-3-RR (AP2S1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 84174-3-RR

  WB result of AP2S1 antibody (84174-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP2S1 transfected HEK-293 cells.

• 

  IHC staining of mouse testis using 84174-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84174-3-RR (AP2S1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 84174-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AP2S1 antibody (84174-3-RR, Clone: 241369F1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84174-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84174-3-RR against Human AP2S1 were performed. The affinity constant is 0.502 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293-Zellen, Maushirngewebe, Mausnierengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84174-3-RR bindet in WB, IHC, IF/ICC, ELISA AP2S1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AP2S1 fusion protein Ag8095
• Vollständiger Name: adaptor-related protein complex 2, sigma 1 subunit
• Berechnetes Molekulargewicht: 142 aa, 17 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC006337
• Gene symbol: AP2S1
• Gene ID (NCBI): 1175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP2S1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes form clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1), and a small adaptin (sigma-type subunit AP2S1). It works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis. Missense mutations of AP2S1 affect Arg15 and lead to familial hypocalciuric hypercalcemia type 3 (FHH3), an extracellular calcium homeostasis disorder affecting the parathyroids, kidneys, and bone (PMID: 23222959).