AP3B1 Polyklonaler Antikörper

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AP3B1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13384-1-PBS

Synonyme

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  WB analysis using 13384-1-AP (same clone as 13384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 13384-1-AP (same clone as 13384-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

• 

  IP experiment of COLO 320 using 13384-1-AP (same clone as 13384-1-PBS)

  IP result of anti-AP3B1 (IP:13384-1-AP, 3ug; Detection:13384-1-AP 1:500) with COLO 320 cells lysate 2500ug. This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 13384-1-AP (same clone as 13384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 13384-1-AP (same clone as 13384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13384-1-PBS bindet in WB, IHC, IP, Indirect ELISA AP3B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP3B1 fusion protein Ag4225
• Vollständiger Name: adaptor-related protein complex 3, beta 1 subunit
• Berechnetes Molekulargewicht: 1094 aa, 121 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC038444
• Gene symbol: AP3B1
• Gene ID (NCBI): 8546
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP3B1 is the 140-kDa β3A subunit of the adaptor-related protein complex-3 (AP-3), a ubiquitous heterotetrameric complex that is localized to the trans-Golgi network and endosomes and is involved in protein trafficking to lysosomes or specialized endosomal-lysosomal organelles (PMID: 9182526; 9545220). This complex is composed of two larger subunits (δ and β3A or β3B), a medium subunit (μ3A or μ3B), and a small subunit (σ3A or σ3B). The absence of the β3A subunit (AP3B1) results in the loss of stability of AP3 and leads to degradation of μ3A, to which β3A is directly bound, while the other subunits are variably affected (PMID: 16507770). AP3B1 contains three main domains: the N-terminal head domain, the hinge, and the C-terminal ear domain. It has been reported as a target of IP(7)-mediated pyrophosphorylation (PMID: 19934039). Defects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) (PMID: 10024875; 16507770).