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AP3B2 Polyklonaler Antikörper

AP3B2 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18331-1-AP

Synonyme

AP 3 complex subunit beta 2, AP3B2, Beta 3B adaptin, NAPTB

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of rat brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    WB analysis of rat brain using 18331-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 18331-1-AP (AP3B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    WB analysis of mouse brain using 18331-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18331-1-AP (AP3B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    IP experiment of mouse brain using 18331-1-AP

    IP Result of anti-AP3B2 (IP:18331-1-AP, 4ug; Detection:18331-1-AP 1:500) with mouse brain tissue lysate 3600ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRattenhirngewebe, Maushirngewebe
Erfolgreiche IPMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below
IFSee 1 publications below

Produktinformation

18331-1-AP bindet in WB, IP, IF, ELISA AP3B2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen AP3B2 fusion protein Ag12861
Vollständiger Name adaptor-related protein complex 3, beta 2 subunit
Berechnetes Molekulargewicht 1082 aa, 119 kDa
Beobachtetes Molekulargewicht 120-140 kDa
GenBank-ZugangsnummerBC093739
Gene symbol AP3B2
Gene ID (NCBI) 8120
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3B2 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Protokolle

Produktspezifische Protokolle
WB protocol for AP3B2 antibody 18331-1-APProtokoll herunterladen
IP protocol for AP3B2 antibody 18331-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Neurology

Autoimmune gait disturbance accompanying adaptor protein-3B2-IgG.

Authors - Josephe A Honorat
View Article