AP3D1 Polyklonaler Antikörper

AP3D1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16454-1-PBS

Synonyme

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  WB analysis of HepG2 using 16454-1-AP (same clone as 16454-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 16454-1-AP (same clone as 16454-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 16454-1-AP (same clone as 16454-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AP3D1 antibody (16454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16454-1-PBS bindet in WB, IF/ICC, Indirect ELISA AP3D1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP3D1 fusion protein Ag9390
• Vollständiger Name: adaptor-related protein complex 3, delta 1 subunit
• Berechnetes Molekulargewicht: 1153 aa, 130 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC010065
• Gene symbol: AP3D1
• Gene ID (NCBI): 8943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.