AP3M1 Polyklonaler Antikörper

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• KD/KO Validated

AP3M1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12114-1-AP

Synonyme

AP 3 complex subunit mu 1, AP3M1, Mu adaptin 3A, Mu3A adaptin

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Galerie der Validierungsdaten

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  WB analysis using 12114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12114-1-AP (AP3M1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12114-1-AP (AP3M1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 12114-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12114-1-AP (AP3M1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 12114-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12114-1-AP (AP3M1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12114-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AP3M1 antibody (12114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

12114-1-AP bindet in WB, IHC, IF, ELISA AP3M1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP3M1 fusion protein Ag2759
• Vollständiger Name: adaptor-related protein complex 3, mu 1 subunit
• Berechnetes Molekulargewicht: 418 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC026232
• Gene symbol: AP3M1
• Gene ID (NCBI): 26985
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Publikationen

Species	Application	Title
human	WB	Elife; A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network.; Authors - Guo Yusong Y ; KD Validated
rat	WB	J Ethnopharmacol; Zhachong Shisanwei Pill resists ischemic stroke by lysosome pathway based on proteomics and bioinformatics; Authors - Qi Song

Verwandte Produkte

AP3D1 Polyclonal antibody

16454-1-AP

AP3B1 Polyclonal antibody

13384-1-AP

AP3B2 Polyclonal antibody

18331-1-AP

AP3M2 Polyclonal antibody

11925-1-AP

AP3S2 Polyclonal antibody

15319-1-AP