AP4B1 Rekombinanter Antikörper

AP4B1 Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

242121D4

Kat-Nr. : 84710-3-RR

Synonyme

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

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  WB analysis using 84710-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84710-3-RR (AP4B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 84710-3-RR

  IP result of anti-AP4B1 (IP:84710-3-RR, 4ug; Detection:84710-3-RR 1:1500) with HeLa cells lysate 1200 ug.

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  Affinity and Kinetic Characterization of 84710-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84710-3-RR against Human AP4B1 were performed. The affinity constant is 47.1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, HeLa-Zellen, HuH-7-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84710-3-RR bindet in WB, IP, ELISA AP4B1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AP4B1 fusion protein Ag9348
• Vollständiger Name: adaptor-related protein complex 4, beta 1 subunit
• Berechnetes Molekulargewicht: 739 aa, 83 kDa
• Beobachtetes Molekulargewicht: ~75 kDa
• GenBank-Zugangsnummer: BC014146
• Gene symbol: AP4B1
• Gene ID (NCBI): 10717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.