AP50 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AP50 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27355-1-PBS

Synonyme

AP2M1, Adaptin mu2, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu

---

• 

  WB analysis using 27355-1-AP (same clone as 27355-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 27355-1-AP (same clone as 27355-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 27355-1-AP (same clone as 27355-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 27355-1-AP (same clone as 27355-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 27355-1-AP (same clone as 27355-1-PBS)

  IP result of anti-AP50 (IP:27355-1-AP, 4ug; Detection:27355-1-AP 1:1000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 27355-1-AP (same clone as 27355-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27355-1-AP (AP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 27355-1-AP (same clone as 27355-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27355-1-AP (AP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

27355-1-PBS bindet in WB, IHC, IP, Indirect ELISA AP50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP50 fusion protein Ag26059
• Vollständiger Name: adaptor-related protein complex 2, mu 1 subunit
• Berechnetes Molekulargewicht: 433 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC013796
• Gene symbol: AP50
• Gene ID (NCBI): 1173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.