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APAF1 Polyklonaler Antikörper

APAF1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29022-1-PBS

Synonyme

APAF 1, APAF-1, Apoptotic protease-activating factor 1, CED4, KIAA0413

Filter:
  • Western Blot (WB) analysis of COLO 320 cells using APAF1 Polyclonal antibody (29022-1-AP)

    WB analysis of COLO 320 using 29022-1-AP (same clone as 29022-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 29022-1-AP (APAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29022-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29022-1-PBS bindet in WB, Indirect ELISA APAF1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen APAF1 fusion protein Ag30606
Vollständiger Name apoptotic peptidase activating factor 1
Berechnetes Molekulargewicht 1248 aa, 142 kDa
Beobachtetes Molekulargewicht130-140 kDa
GenBank-ZugangsnummerBC136532
Gene symbol APAF1
Gene ID (NCBI) 317
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.