APCDD1 Rekombinanter Antikörper

APCDD1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240085C6

Kat-Nr. : 83246-6-RR

Synonyme

Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein

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  WB analysis using 83246-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83246-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83246-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83246-6-RR against Human APCDD1 were performed. The affinity constant is 0.634 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen, Mausherzgewebe, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83246-6-RR bindet in WB, ELISA APCDD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: APCDD1 fusion protein Ag10303
• Vollständiger Name: adenomatosis polyposis coli down-regulated 1
• Berechnetes Molekulargewicht: 514 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC053324
• Gene symbol: APCDD1
• Gene ID (NCBI): 147495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APCDD1(adenomatosis polyposis coli down-regulated 1) is a membrane-bound glycoprotein conserved during vertebrate evolution that regulates important biological processes controlled by Wnt signaling (PMID: 26170146). APCDD1 is a membrane-bound glycoprotein that is abundantly expressed in human HFs (hair follicles) and can interact in vitro with WNT3A and LRP5, two essential components of Wnt signaling (PMID: 20393562). APCDD1 may be modified by glycosylation.