Serum amyloid P component Polyklonaler Antikörper

Serum amyloid P component Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20773-1-AP

Synonyme

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

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  WB analysis of human kidney using 20773-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20773-1-AP bindet in WB, IHC, ELISA Serum amyloid P component und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Serum amyloid P component fusion protein Ag14765
• Vollständiger Name: amyloid P component, serum
• Berechnetes Molekulargewicht: 223 aa, 25 kDa
• Beobachtetes Molekulargewicht: 27-29 kDa
• GenBank-Zugangsnummer: BC007058
• Gene symbol: Serum amyloid P component
• Gene ID (NCBI): 325
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Publikationen

Species	Application	Title
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
human	WB	JACC Basic Transl Sci; Bioprosthetic Valve Deterioration: Accumulation of Circulating Proteins and Macrophages in the Valve Interstitium; Authors - Tomohisa Sakaue