APEX1 Rekombinanter Antikörper

APEX1 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251693G12

Kat-Nr. : 86719-1-PBS

Synonyme

APE, APE1, APE-1, APEN, APEX

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  WB analysis using 86719-1-RR (same clone as 86719-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86719-1-RR (APEX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86719-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02621-2

  Plasma of six individual healthy human donors was measured. The APEX1 concentration of detected samples was determined to be 0.22 ng/mL with a range of 0.11-0.41 ng/mL.

• 

  Sandwich ELISA sample test of MP02621-2

  The mean APEX1 concentration was determined to be 141.21 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load and 137.64 ng/mL in HCT 116 cell extract based on a 1.30 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02621-2

  Sandwich ELISA standard curve of MP02621-2, Human APEX1 Recombinant Matched Antibody Pair - PBS only. 86719-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0251. 86719-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86719-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA APEX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: APEX1 fusion protein Ag0251
• Vollständiger Name: APEX nuclease (multifunctional DNA repair enzyme) 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002338
• Gene symbol: APEX1
• Gene ID (NCBI): 328
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression.