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APEX1 Polyklonaler Antikörper
APEX1 Polyklonal Antikörper für IF/ICC, FC (Intra)
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
IF/ICC, FC (Intra)
Konjugation
CoraLite® Plus 488 Fluorescent Dye
Kat-Nr. : CL488-10203
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in IF/ICC | MCF-7-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL488-10203 bindet in IF/ICC, FC (Intra) APEX1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | APEX1 fusion protein Ag0251 |
Vollständiger Name | APEX nuclease (multifunctional DNA repair enzyme) 1 |
Berechnetes Molekulargewicht | 36 kDa |
Beobachtetes Molekulargewicht | 36 kDa |
GenBank-Zugangsnummer | BC002338 |
Gene symbol | APEX1 |
Gene ID (NCBI) | 328 |
Konjugation | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10203-1-AP is a rabbit polyclonal antibody raised against full length APE1 of human origin.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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IF protocol for CL Plus 488 APEX1 antibody CL488-10203 | Protokoll herunterladen |
FC protocol for CL Plus 488 APEX1 antibody CL488-10203 | Download protocol |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |