APH1A Polyklonaler Antikörper

APH1A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11643-1-PBS

Synonyme

CGI-78, CGI 78, Aph-1alpha, APH-1a, Aph 1alpha

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  WB analysis using 11643-1-AP (same clone as 11643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11643-1-AP (same clone as 11643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11643-1-AP (same clone as 11643-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

  IP result of anti-APH1A (IP:11643-1-AP, 4ug; Detection:11643-1-AP 1:4000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 11643-1-AP (same clone as 11643-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 11643-1-AP (same clone as 11643-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APH1A antibody (11643-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11643-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA APH1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APH1A fusion protein Ag2238
• Vollständiger Name: anterior pharynx defective 1 homolog A (C. elegans)
• Berechnetes Molekulargewicht: 265 aa, 29 kDa
• Beobachtetes Molekulargewicht: 27-32 kDa
• GenBank-Zugangsnummer: BC015568
• Gene symbol: APH1A
• Gene ID (NCBI): 51107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.