API5 Polyklonaler Antikörper

API5 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 25689-1-AP

Synonyme

AAC 11, AAC11, Antiapoptosis clone 11 protein, API 5, API5, apoptosis inhibitor 5, FIF, Protein XAGL

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Galerie der Validierungsdaten

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  WB analysis using 25689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25689-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25689-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25689-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 25689-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25689-1-AP (API5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 25689-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25689-1-AP (API5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25689-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using API5 antibody (25689-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 25689-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25689-1-AP (API5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

25689-1-AP bindet in WB, IHC, IF, ELISA API5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: API5 fusion protein Ag22524
• Vollständiger Name: apoptosis inhibitor 5
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC017709
• Gene symbol: API5
• Gene ID (NCBI): 8539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human gene API5 encodes the protein Apoptosis inhibitor 5. This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of chemotherapeutic drugs. Diseases associated with API5 include colon adenocarcinoma, and cervical cancer.

Publikationen

Species	Application	Title
mouse	IHC	Sci Rep; microRNA regulatory circuits in a mouse model of inherited retinal degeneration.; Authors - Arpad Palfi
	WB	Gene; Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica.; Authors - Shuang Zhang
human	WB	Oncol Lett; Paeonol-mediated apoptosis of hepatocellular carcinoma cells by NF-κB pathway.; Authors - Qiang Li