Apolipoprotein A II Polyklonaler Antikörper

Apolipoprotein A II Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16845-1-AP

Synonyme

Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2

---

Galerie der Validierungsdaten

• 

  WB analysis of human plasma using 16845-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 16845-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 16845-1-AP

  IP result of anti-Apolipoprotein A II (IP:16845-1-AP, 4ug; Detection:16845-1-AP 1:500) with human plasma lysate 4000 ug.

• 

  IHC staining of human liver using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16845-1-AP (Apolipoprotein A II antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16845-1-AP (Apolipoprotein A II antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16845-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

16845-1-AP bindet in WB, IP, IHC, IF, ELISA Apolipoprotein A II und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Apolipoprotein A II fusion protein Ag9863
• Vollständiger Name: apolipoprotein A-II
• Berechnetes Molekulargewicht: 100 aa, 11 kDa
• Beobachtetes Molekulargewicht: 7-9 kDa (monomer), 15 kDa (dimer)
• GenBank-Zugangsnummer: BC005282
• Gene symbol: APOA2
• Gene ID (NCBI): 336
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

Publikationen

Species	Application	Title
human	WB	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
human	WB	Exp Cell Res; Extracellular vesicle-mediated transfer of the lncRNA-TC0101441 promotes endometriosis migration/invasion.; Authors - Jun-Jun Qiu
human,mouse	WB	Int J Mol Sci; Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes; Authors - Zhonghao Li