APOA4 Monoklonaler Antikörper

APOA4 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A9A8

Kat-Nr. : 66252-1-PBS

Synonyme

3A9A8, APOA 4, apolipoprotein A IV

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  WB analysis of human plasma using 66252-1-Ig (same clone as 66252-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66252-1-Ig (same clone as 66252-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APOA4 antibody (66252-1-Ig, Clone: 3A9A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66252-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA APOA4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: APOA4 fusion protein Ag12454
• Vollständiger Name: apolipoprotein A-IV
• Berechnetes Molekulargewicht: 396 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-46 kDa
• GenBank-Zugangsnummer: BC074764
• Gene symbol: APOA4
• Gene ID (NCBI): 337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.