APOB Polyklonaler Antikörper

APOB Polyklonal Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB

Konjugation

HRP

Kat-Nr. : HRP-20578

Synonyme

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslebergewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

HRP-20578 bindet in WB APOB und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name apolipoprotein B (including Ag(x) antigen)
Berechnetes Molekulargewicht 516 kDa
Beobachtetes Molekulargewicht150-250 kDa, 400-520 kDa
GenBank-ZugangsnummerNM_000384
Gene symbol APOB
Gene ID (NCBI) 338
Konjugation HRP
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 and APOB-27.6 (PMID:11839763, PMID:2450346).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HRP APOB antibody HRP-20578Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tony (Verified Customer) (08-28-2025)

The Western Blot shows a band corresponding to APOB, detected using the HRP-conjugated polyclonal antibody. The signal intensity confirms the effective recognition of the target protein.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12