APOBEC3A Rekombinanter Antikörper

APOBEC3A Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241529F1

Kat-Nr. : 84239-2-RR

Synonyme

PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A

---

• 

  IF Staining of THP-1 using 84239-2-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using APOBEC3A antibody (84239-2-RR, Clone: 241529F1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84239-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84239-2-RR against Human APOBEC3A were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84239-2-RR bindet in IF/ICC, ELISA APOBEC3A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: APOBEC3A fusion protein Ag18845
• Vollständiger Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
• Berechnetes Molekulargewicht: 199 aa, 23 kDa
• GenBank-Zugangsnummer: BC126416
• Gene symbol: APOBEC3A
• Gene ID (NCBI): 200315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.