APOBEC3G Polyklonaler Antikörper

APOBEC3G Polyklonal Antikörper für WB, IP, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 10608-1-AP

Synonyme

A3G, APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

---

• 

  WB analysis of mouse colon using 10608-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10608-1-AP (APOBEC3G Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 10608-1-AP

  IP result of anti-APOBEC3G (IP:10608-1-AP, 4ug; Detection:10608-1-AP 1:300) with COLO 320 cells lysate 2240ug.

• 

  IHC staining of human testis using 10608-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10608-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10608-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 10608-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 10608-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10608-1-AP (APOBEC3G antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

10608-1-AP bindet in WB, IP, IF, IHC, ELISA APOBEC3G und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOBEC3G fusion protein Ag0887
• Vollständiger Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC024268
• Gene symbol: APOBEC3G
• Gene ID (NCBI): 60489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB,IHC	J Clin Invest; APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis.; Authors - Ding Qingqing Q
human	WB	Cancers (Basel); Overexpression of NNMT in Glioma Aggravates Tumor Cell Progression: An Emerging Therapeutic Target; Authors - Wei Sun