APOD Polyklonaler Antikörper

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• KD/KO Validated

APOD Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10520-1-AP

Synonyme

Apo D, APOD, apolipoprotein D

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 10520-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 10520-1-AP (APOD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10520-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10520-1-AP (APOD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 10520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10520-1-AP (APOD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10520-1-AP (APOD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 10520-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human APOD (10520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Serumgewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

10520-1-AP bindet in WB, IHC, FC, ELISA APOD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOD fusion protein Ag0812
• Vollständiger Name: apolipoprotein D
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 21-33 kDa
• GenBank-Zugangsnummer: BC007402
• Gene symbol: APOD
• Gene ID (NCBI): 347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.

Publikationen

Species	Application	Title
human	WB,IHC	Neuropathol Appl Neurobiol; Apolipoprotein D: a potential biomarker for cerebral amyloid angiopathy.; Authors - H Bea Kuiperij
human	WB	Clin Proteomics; Integration of transcriptome and proteome profiles in placenta accreta reveals trophoblast over-migration as the underlying pathogenesis.; Authors - Na Li ; KD Validated
human	WB,IHC	Biomarkers; Apolipoprotein D as a novel marker in human end-stage heart failure: a preliminary study.; Authors - Wei Ying-Jie YJ
human	IHC	Front Immunol; A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma; Authors - Muhammad Khan