APOD Monoklonaler Antikörper

APOD Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6D10

Kat-Nr. : 66215-1-PBS

Synonyme

1C6D10, Apo D, Apo-D, apolipoprotein D

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  WB analysis of human plasma using 66215-1-Ig (same clone as 66215-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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  WB analysis using 66215-1-Ig (same clone as 66215-1-PBS)

  human urine sample were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66215-1-Ig (same clone as 66215-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66215-1-Ig (same clone as 66215-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using APOD antibody (66215-1-Ig, Clone: 1C6D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66215-1-Ig (same clone as 66215-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APOD (66215-1-Ig, Clone:1C6D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66215-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA APOD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: APOD fusion protein Ag21422
• Vollständiger Name: apolipoprotein D
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC007402
• Gene symbol: APOD
• Gene ID (NCBI): 347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.