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APOE Rekombinanter Antikörper

APOE Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242310B2

Kat-Nr. : 84775-7-PBS

Synonyme

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

Filter:
  • Immunohistochemistry (IHC) staining of mouse liver tissue using APOE Recombinant antibody (84775-7-RR)

    IHC staining of mouse liver using 84775-7-RR (same clone as 84775-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using APOE Recombinant antibody (84775-7-RR)

    IHC staining of mouse liver using 84775-7-RR (same clone as 84775-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOE Recombinant antibody (84775-7-RR)

    IF Staining of HepG2 using 84775-7-RR (same clone as 84775-7-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (84775-7-RR, Clone: 242310B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84775-7-RR

    Affinity and Kinetic Characterization of 84775-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84775-7-RR against Human APOE were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84775-7-PBS bindet in IHC, IF/ICC, Indirect ELISA APOE und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name Apolipoprotein E
Berechnetes Molekulargewicht 36 kDa
GenBank-ZugangsnummerBC003557
Gene symbol APOE
Gene ID (NCBI) 348
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).