APOE Monoklonaler Antikörper

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• KD/KO Validated

APOE Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

1B2C9

Kat-Nr. : CL555-66830

Synonyme

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

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  IF Staining of Caco-2 using CL555-66830

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite®555 APOE antibody (CL555-66830, Clone: 1B2C9 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL555-66830

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated APOE Monoclonal antibody (CL555-66830, Clone:1B2C9)(red), or 0.4 ug CoraLite®555 Mouse IgG1 Isotype Control (MOPC-21) (CL555-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66830 bindet in IF/ICC, FC (Intra) APOE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: APOE fusion protein Ag28186
• Vollständiger Name: Apolipoprotein E
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 34-36 kDa
• GenBank-Zugangsnummer: BC003557
• Gene symbol: APOE
• Gene ID (NCBI): 348
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.