APOH Rekombinanter Antikörper

APOH Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242454E4

Kat-Nr. : 84943-2-RR

Synonyme

Apolipoprotein H, 242454E4, Activated protein C-binding protein, Apo H, Apo-H

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  WB analysis of human plasma using 84943-2-RR

  human plasma were subjected to SDS PAGE followed by western blot with 84943-2-RR (APOH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 84943-2-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84943-2-RR (APOH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 84943-2-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84943-2-RR (APOH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84943-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84943-2-RR against Human APOH were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84943-2-RR bindet in WB, IHC, ELISA APOH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: APOH fusion protein Eg3084
• Vollständiger Name: apolipoprotein H (beta-2-glycoprotein I)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 43-55 kDa
• GenBank-Zugangsnummer: NM_000042.2
• Gene symbol: APOH
• Gene ID (NCBI): 350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.