APOL1-Specific Monoklonaler Antikörper

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• KD/KO Validated

APOL1-Specific Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G12D11

Kat-Nr. : 66124-1-PBS

Synonyme

APOL1, 1G12D11, Apo-L, ApoL-I, Apolipoprotein L I

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  WB analysis of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

  1.5 ug human plasma was subjected to SDS PAGE followed by western blot with 66124-1-Ig (APOL1 Antibody) at dilution of 1:400, 000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

  IP result of anti-APOL1-Specific (IP:66124-1-Ig, 5ug; Detection:66124-1-Ig 1:10000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL1-Specific antibody (66124-1-Ig, Clone: 1G12D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66124-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66124-1-Ig against Human APOL1 were performed. The Affinity Constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

66124-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA APOL1-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: APOL1-Specific fusion protein Ag2016
• Vollständiger Name: apolipoprotein L, 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC017331
• Gene symbol: APOL1
• Gene ID (NCBI): 8542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.