APOM Polyklonaler Antikörper

APOM Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12817-1-AP

Synonyme

Apo M, Apolipoprotein M, Apo-M, G3A, HSPC336

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  WB analysis using 12817-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 12817-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12817-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12817-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 12817-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 12817-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12817-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (12817-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, human plasma
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

12817-1-AP bindet in WB, IHC, IF/ICC, ELISA APOM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOM fusion protein Ag3137
• Vollständiger Name: apolipoprotein M
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC020683
• Gene symbol: APOM
• Gene ID (NCBI): 55937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).

Publikationen

Species	Application	Title
human	WB	Apoptosis; The miR-573/apoM/Bcl2A1-dependent signal transduction pathway is essential for hepatocyte apoptosis and hepatocarcinogenesis.; Authors - Yan-Wei Hu
mouse	WB	J Biol Chem; Effect of Phospholipid Transfer Protein on Plasma Sphingosine-1-Phosphate; Authors - Quiana Jones ; KO Validated