APOM Polyklonaler Antikörper

APOM Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12817-1-PBS

Synonyme

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  WB analysis using 12817-1-AP (same clone as 12817-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 12817-1-AP (same clone as 12817-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12817-1-AP (same clone as 12817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 12817-1-AP (same clone as 12817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 12817-1-AP (same clone as 12817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hepatocirrhosis using 12817-1-AP (same clone as 12817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12817-1-AP (same clone as 12817-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (12817-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12817-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA APOM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOM fusion protein Ag3137
• Vollständiger Name: apolipoprotein M
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC020683
• Gene symbol: APOM
• Gene ID (NCBI): 55937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).