APOOL Polyklonaler Antikörper

APOOL Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28514-1-PBS

Synonyme

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  WB analysis using 28514-1-AP (same clone as 28514-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28514-1-AP (APOOL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 28514-1-AP (same clone as 28514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 28514-1-AP (same clone as 28514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 28514-1-AP (same clone as 28514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 28514-1-AP (same clone as 28514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 28514-1-AP (same clone as 28514-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using APOOL antibody (28514-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28514-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA APOOL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOOL fusion protein Ag29392
• Vollständiger Name: apolipoprotein O-like
• Berechnetes Molekulargewicht: 268 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC107096
• Gene symbol: APOOL
• Gene ID (NCBI): 139322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOOL, the component of the MICOS complex, is a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:23704930), (PubMed:25764979). Its molecular weight is about 30 kDa.