APP Rekombinanter Antikörper

APP Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250546B3

Kat-Nr. : 84829-6-PBS

Synonyme

amyloid precursor protein, A4 amyloid protein, AB40, AB42, AD1

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  WB analysis using 84829-6-RR (same clone as 84829-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84829-6-RR (APP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84829-6-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 84829-6-RR (same clone as 84829-6-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84829-6-RR (APP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84829-6-PBS in a different storage buffer formulation.

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  Sample test of MP01584-4

  The mean APP concentration was determined to be 151.0 ng/mL in human serum.

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  IF Staining of U-251 using 84829-6-RR (same clone as 84829-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using APP antibody (84829-6-RR, Clone: 250546B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84829-6-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01584-4

  Cytometric bead array standard curve of MP01584-4, APP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84829-7-PBS. Detection antibody: 84829-6-PBS. Standard: Eg3070. Range: 0.625-80 ng/mL.

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Geprüfte Anwendungen

Produktinformation

84829-6-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test APP und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: APP fusion protein Eg3070
• Vollständiger Name: amyloid beta (A4) precursor protein
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_000484.3
• Gene symbol: APP
• Gene ID (NCBI): 351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).