APPL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

APPL1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (3)

Kat-Nr. : 12639-1-AP

Synonyme

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428

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Galerie der Validierungsdaten

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  WB analysis using 12639-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12639-1-AP (APPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12639-1-AP

  WB result of APPL1 antibody (12639-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

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  WB analysis of mouse ovary using 12639-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12639-1-AP (APPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12639-1-AP

  IP Result of anti-APPL1 (IP:12639-1-AP, 4ug; Detection:12639-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 12639-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12639-1-AP (APPL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 12639-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (12639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 12639-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (12639-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, HEK-293-Zellen, HeLa-Zellen, Maus-Eierstockgewebe, Mauslebergewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

12639-1-AP bindet in WB, IP, IHC, IF, FC, ELISA APPL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APPL1 fusion protein Ag3334
• Vollständiger Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
• Berechnetes Molekulargewicht: 709 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC028599
• Gene symbol: APPL1
• Gene ID (NCBI): 26060
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

Publikationen

Species	Application	Title
rat	WB	Oxid Med Cell Longev; DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway.; Authors - Yichen Zhao
human	IF	J Cell Sci; ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome.; Authors - Neftali Flores-Rodriguez
human	WB	J Biol Chem; Endocytosis is required for C-X-C chemokine receptor type 4 (CXCR4)-mediated Akt activation and anti-apoptotic signaling.; Authors - Elizabeth J English ; KD Validated
mouse	WB	J Agric Food Chem; Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.; Authors - Peiyuan Li
mouse	WB,IF	Biol Reprod; Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.; Authors - Przemysław Zakrzewski
human	WB,IHC	Brain Res; Characterization and distribution of adaptor protein containing a PH domain, PTB domain and leucine zipper motif (APPL1) in Alzheimer's disease hippocampus: an immunohistochemical study.; Authors - Akari Ogawa

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ying (Verified Customer) (09-11-2023)

used for WB and IFC of brain section of wild type and APPL1 transgenic mice

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:500 for IFC, 1:1000 for WB
• Cell Tissue Type: mouse brain/human fibroblast

APPL1 Antibody Western Blot,Immunohistochemistry,Immunofluorescence,Immunoprecipitation validation (1:500 for IFC, 1:1000 for WB dilution) in mouse brain/human fibroblast (Cat no:12639-1-AP)

FH

Sophie (Verified Customer) (11-11-2022)

I used the antibody in a western blot, my samples were primary B-cell lysates (10ug/lane). The antibody did label APPL1 around the 80kDa mark, but I did see another band lower down, around 37kDa - maybe this is a cleavage product?

• Applications: Western Blot
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: Primary B-cells (human, from blood)

APPL1 Antibody Western Blot validation (1:2,000 dilution) in Primary B-cells (human, from blood) (Cat no:12639-1-AP)

FH

X (Verified Customer) (01-18-2021)

Very strong and specific signal in WB with mouse brain lysate

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse brain

APPL1 Antibody Western Blot validation (1:5000 dilution) in mouse brain (Cat no:12639-1-AP)