APPL1 Polyklonaler Antikörper

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• KD/KO Validated

APPL1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12639-1-PBS

Synonyme

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  WB analysis using 12639-1-AP (same clone as 12639-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12639-1-AP (APPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12639-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12639-1-AP (same clone as 12639-1-PBS)

  WB result of APPL1 antibody (12639-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 12639-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 12639-1-AP (same clone as 12639-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12639-1-AP (APPL1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12639-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12639-1-AP (same clone as 12639-1-PBS)

  IP result of anti-APPL1 (IP:12639-1-AP, 4ug; Detection:12639-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12639-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12639-1-AP (same clone as 12639-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12639-1-AP (APPL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12639-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12639-1-AP (same clone as 12639-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (12639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12639-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 12639-1-AP (same clone as 12639-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (12639-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12639-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12639-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA APPL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APPL1 fusion protein Ag3334
• Vollständiger Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
• Berechnetes Molekulargewicht: 709 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC028599
• Gene symbol: APPL1
• Gene ID (NCBI): 26060
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].