APPL2 Polyklonaler Antikörper

APPL2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 14294-1-AP

Synonyme

Dip13-beta, DIP13B, Dip13 beta, DCC-interacting protein 13-beta, Adapter protein containing PH domain, PTB domain and leucine zipper motif 2

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  WB analysis of BxPC-3 using 14294-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 14294-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14294-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14294-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 14294-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14294-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 14294-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using APPL2 antibody (14294-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes malignes Melanomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

14294-1-AP bindet in WB, IHC, IF/ICC, ELISA APPL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APPL2 fusion protein Ag5626
• Vollständiger Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC033731
• Gene symbol: APPL2
• Gene ID (NCBI): 55198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Cachexia Sarcopenia Muscle; Physical exercise attenuates age-related muscle atrophy and exhibits anti-ageing effects via the adiponectin receptor 1 signalling; Authors - Yuan-Li Chen
human	WB	J Immunol; Long Noncoding RNA HAFML Promotes Migration and Invasion of Rheumatoid Fibroblast-like Synoviocytes; Authors - Siqi Xu
mouse	IF	J Cell Sci; GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region; Authors - Paroma Chatterjee
mouse	IF	bioRxiv; GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region; Authors - Paroma Chatterjee