APRIL,TNFSF13 Polyklonaler Antikörper

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• KD/KO Validated

APRIL,TNFSF13 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 10680-1-AP

Synonyme

TNFSF13, TNF- and APOL-related leukocyte expressed ligand 2, TALL-2, TALL2, CD256

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  WB analysis of Raji using 10680-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10680-1-AP (TNFSF13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10680-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10680-1-AP (TNFSF13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10680-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10680-1-AP (TNFSF13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 10680-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10680-1-AP (TNFSF13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

10680-1-AP bindet in WB, IHC, ELISA APRIL,TNFSF13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APRIL,TNFSF13 fusion protein Ag1056
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 13
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC008042
• Gene symbol: TNFSF13
• Gene ID (NCBI): 8741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFSF13 gene encodes tumor necrosis factor ligand superfamily member 13 (or APRIL) belonging to the tumor necrosis factor (TNF) family. APRIL is highly expressed in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages, and its precursor is cleaved by furin. APRIL may be implicated in the regulation of tumor cell growth and also monocyte/macrophage-mediated immunological processes.

Publikationen

Species	Application	Title
human	IHC	Front Immunol; TNFSF13 Is a Novel Onco-Inflammatory Marker and Correlates With Immune Infiltration in Gliomas.; Authors - Rui Chen
human	WB	Clin Biochem; APRIL knockdown suppresses migration and invasion of human colon carcinoma cells.; Authors - Ding Weifeng W ; KD Validated
human	WB	Pathol Oncol Res; Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway.; Authors - Ding Weifeng W ; KD Validated