APRIL/TNFSF13 Rekombinanter Antikörper

APRIL/TNFSF13 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241666F9

Kat-Nr. : 98331-2-PBS

Synonyme

241666F9, A proliferation-inducing ligand, APRIL, CD256, Tnfsf13

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  FC experiment of Transfected HEK-293T using 98331-2-RR (same clone as 98331-2-PBS)

  1x10^6 HEK-293T cells or APRIL/TNFSF13 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse APRIL/TNFSF13 Rabbit RecAb (98331-2-RR, Clone: 241666F9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98331-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293T using 98331-2-RR (same clone as 98331-2-PBS)

  1x10^6 HEK-293T cells or APRIL/TNFSF13 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse APRIL/TNFSF13 Rabbit RecAb (98331-2-RR, Clone:241666F9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98331-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98331-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98331-2-RR against Mouse APRIL/TNFSF13 were performed. The affinity constant is 0.228 nM.

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Geprüfte Anwendungen

Produktinformation

98331-2-PBS bindet in FC (Intra) APRIL/TNFSF13 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: APRIL/TNFSF13 fusion protein Eg1561
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 13
• Berechnetes Molekulargewicht: 27kDa
• GenBank-Zugangsnummer: NM_023517.2
• Gene symbol: Tnfsf13
• Gene ID (NCBI): 69583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

TNFSF13 gene encodes tumor necrosis factor ligand superfamily member 13 (or APRIL) belonging to the tumor necrosis factor (TNF) family. APRIL is highly expressed in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages, and its precursor is cleaved by furin. APRIL may be implicated in the regulation of tumor cell growth and also monocyte/macrophage-mediated immunological processes.