AQP1 Monoklonaler Antikörper

AQP1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E5D6

Kat-Nr. : 66805-1-PBS

Synonyme

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

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  WB analysis of human heart using 66805-1-Ig (same clone as 66805-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66805-1-Ig (same clone as 66805-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AQP1 antibody (66805-1-Ig, Clone: 2E5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66805-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA AQP1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AQP1 fusion protein Ag14093
• Vollständiger Name: aquaporin 1 (Colton blood group)
• Berechnetes Molekulargewicht: 269 aa, 29 kDa
• Beobachtetes Molekulargewicht: 28 kDa, 38-40 kDa
• GenBank-Zugangsnummer: BC022486
• Gene symbol: AQP1
• Gene ID (NCBI): 358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)