AQP2 Rekombinanter Antikörper

AQP2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242886B8

Kat-Nr. : 85402-1-RR

Synonyme

Aquaporin 2, AQP-2, Aquaporin2

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  WB analysis using 85402-1-RR

  Unboiled mouse kidney tissue were subjected to SDS PAGE followed by western blot with 85402-1-RR (AQP2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 85402-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85402-1-RR (AQP2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 85402-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85402-1-RR (AQP2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85402-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85402-1-RR against Human AQP2 were performed. The affinity constant is 0.955 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, unboiled mouse kidney tissue
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85402-1-RR bindet in WB, IHC, ELISA AQP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AQP2 fusion protein Ag29931
• Vollständiger Name: aquaporin 2 (collecting duct)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 28-29 kDa, 35-38 kDa
• GenBank-Zugangsnummer: NM_000486
• Gene symbol: AQP2
• Gene ID (NCBI): 359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.