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Aquaporin 4 Polyklonaler Antikörper

Aquaporin 4 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(117)

Kat-Nr. : 16473-1-AP

Synonyme

AQP4, AQP 4, Mercurial-insensitive water channel, Aquaporin-4, Aquaporin4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslebergewebe, Maushirngewebe, Mausherzgewebe, Mausnierengewebe, Rattenhirngewebe, Rattenherzgewebe, SKOV-3-Zellen
Erfolgreiche IPMausherzgewebe
Erfolgreiche Detektion in IHCMaushirngewebe, humanes Hirngewebe, humanes Nierengewebe, Mausherzgewebe, Rattenhirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PRattenhirngewebe, Maushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:100-1:2500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16473-1-AP bindet in WB, IHC, IF-P, IP, ELISA Aquaporin 4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Aquaporin 4 fusion protein Ag9561
Vollständiger Name Aquaporin 4
Berechnetes Molekulargewicht 323 aa, 35 kDa
Beobachtetes Molekulargewicht 35-37 kDa, 32-34 kDa
GenBank-ZugangsnummerBC022286
Gene symbol Aquaporin 4
Gene ID (NCBI) 361
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Aquaporin 4 antibody 16473-1-APProtokoll herunterladen
IHC protocol for Aquaporin 4 antibody 16473-1-APProtokoll herunterladenl
IF protocol for Aquaporin 4 antibody 16473-1-APProtokoll herunterladen
IP protocol for Aquaporin 4 antibody 16473-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

J Clin Invest

MicroRNA-26a-3p rescues depression-like behaviors in male rats via preventing hippocampal neuronal anomalies.

Authors - Ye Li
humanIF

Clin Cancer Res

A Histopathologic Correlation Study Evaluating Glymphatic Function in Brain Tumors by Multi-Parametric MRI

Authors - Min Gao
humanIF

Brain

Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia.

Authors - Aiqing Chen
mouseWB,IF

Brain Behav Immun

Polyunsaturated fatty acid supplement alleviates depression-incident cognitive dysfunction by protecting the cerebrovascular and glymphatic systems.

Authors - Xinghua Liu
mouseWB

Glia

Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice.

Authors - Wenqiang Quan
mouseWB

Brain Behav Immun

Hypoxia augments LPS-induced inflammation and triggers high altitude cerebral edema in mice.

Authors - Yanzhao Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

GARGI (Verified Customer) (08-07-2025)

Nice and clear staining using this antibody

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Neurons
FH

Paula (Verified Customer) (07-30-2024)

Worked well for human primary cell line, dilution used 1:500 (secondary 1:400)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human
Aquaporin 4 Antibody Immunofluorescence validation (1:500 dilution) in Human (Cat no:16473-1-AP)
FH

aidan (Verified Customer) (07-27-2024)

Very happy with this antibody would highly recommend. Overnight 4degrees, 1:2000. Bis tris gel. Secondary 1:4000 HRP 1h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: muller retinal mouse
Aquaporin 4 Antibody Western Blot validation (1:2000 dilution) in muller retinal mouse (Cat no:16473-1-AP)
FH

Reyes (Verified Customer) (04-05-2024)

Aquaporin 4 (in green) shows a strong stainning, although difficult for the analyses, in human FFPE brain cortex.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
Aquaporin 4 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:16473-1-AP)