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Aquaporin 4 Polyklonaler Antikörper
Aquaporin 4 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF-P, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16473-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Mauslebergewebe, Maushirngewebe, Mausherzgewebe, Mausnierengewebe, Rattenhirngewebe, Rattenherzgewebe, SKOV-3-Zellen |
Erfolgreiche IP | Mausherzgewebe |
Erfolgreiche Detektion in IHC | Maushirngewebe, humanes Hirngewebe, humanes Nierengewebe, Mausherzgewebe, Rattenhirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Rattenhirngewebe, Maushirngewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:100-1:2500 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 74 publications below |
IHC | See 23 publications below |
IF | See 49 publications below |
IP | See 1 publications below |
FC | See 1 publications below |
Produktinformation
16473-1-AP bindet in WB, IHC, IF-P, IP, ELISA Aquaporin 4 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Aquaporin 4 fusion protein Ag9561 |
Vollständiger Name | Aquaporin 4 |
Berechnetes Molekulargewicht | 323 aa, 35 kDa |
Beobachtetes Molekulargewicht | 35-37 kDa, 32-34 kDa |
GenBank-Zugangsnummer | BC022286 |
Gene symbol | Aquaporin 4 |
Gene ID (NCBI) | 361 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Aquaporin 4 antibody 16473-1-AP | Protokoll herunterladen |
IHC protocol for Aquaporin 4 antibody 16473-1-AP | Protokoll herunterladenl |
IF protocol for Aquaporin 4 antibody 16473-1-AP | Protokoll herunterladen |
IP protocol for Aquaporin 4 antibody 16473-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Clin Invest MicroRNA-26a-3p rescues depression-like behaviors in male rats via preventing hippocampal neuronal anomalies. | ||
Clin Cancer Res A Histopathologic Correlation Study Evaluating Glymphatic Function in Brain Tumors by Multi-Parametric MRI | ||
Brain Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia. | ||
Brain Behav Immun Polyunsaturated fatty acid supplement alleviates depression-incident cognitive dysfunction by protecting the cerebrovascular and glymphatic systems. | ||
Glia Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice. | ||
Brain Behav Immun Hypoxia augments LPS-induced inflammation and triggers high altitude cerebral edema in mice. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH GARGI (Verified Customer) (08-07-2025) | Nice and clear staining using this antibody
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FH Paula (Verified Customer) (07-30-2024) | Worked well for human primary cell line, dilution used 1:500 (secondary 1:400)
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FH aidan (Verified Customer) (07-27-2024) | Very happy with this antibody would highly recommend. Overnight 4degrees, 1:2000. Bis tris gel. Secondary 1:4000 HRP 1h RT.
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FH Reyes (Verified Customer) (04-05-2024) | Aquaporin 4 (in green) shows a strong stainning, although difficult for the analyses, in human FFPE brain cortex.
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