Aquaporin 4 Monoklonaler Antikörper

Aquaporin 4 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G7D3

Kat-Nr. : 68448-1-PBS

Synonyme

AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4

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  WB analysis using 68448-1-Ig (same clone as 68448-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spinal cord using 68448-1-Ig (same clone as 68448-1-PBS)

  rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68448-1-Ig (same clone as 68448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68448-1-Ig (same clone as 68448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68448-1-PBS bindet in WB, IHC, Indirect ELISA Aquaporin 4 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aquaporin 4 fusion protein Ag9830
• Vollständiger Name: Aquaporin 4
• Berechnetes Molekulargewicht: 323 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC022286
• Gene symbol: Aquaporin 4
• Gene ID (NCBI): 361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.