AQP5 Polyklonaler Antikörper

AQP5 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20334-1-PBS

Synonyme

aquaporin 5, Aquaporin-5, aquaporin5, AQP-5, AQP 5

---

• 

  WB analysis using 20334-1-AP (same clone as 20334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20334-1-AP (AQP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat lung using 20334-1-AP (same clone as 20334-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AQP5 antibody (20334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20334-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA AQP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AQP5 fusion protein Ag14103
• Vollständiger Name: aquaporin 5
• Berechnetes Molekulargewicht: 265 aa, 28 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC032946
• Gene symbol: AQP5
• Gene ID (NCBI): 362
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AQP5 is a member of aquaporins (AQPs) which are membrane proteins functioning as water channels and involved in the bidirectional transfer of water and small solutes across cell membranes. AQP5 is widely expressed including digestive, renal, respiratory, and reproductive systems. AQP5 overexpression in cancer cells and tumor tissues has been extensively reported. Recently AQP5 has been identified as a marker for adult pyloric stem cells.