AQP6 Rekombinanter Antikörper

AQP6 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240175F9

Kat-Nr. : 83322-3-RR

Synonyme

Aquaporin6, Aquaporin 6, AQP 6, 240175F9

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  WB analysis using 83322-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83322-3-RR (AQP6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83322-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83322-3-RR against Human AQP6 were performed. The affinity constant is 0.749 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, MCF-7-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83322-3-RR bindet in WB, ELISA AQP6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AQP6 fusion protein Ag26845
• Vollständiger Name: aquaporin 6, kidney specific
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: NM_001652
• Gene symbol: AQP6
• Gene ID (NCBI): 363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AQP6 (Aquaporin-6) has recently been identified as an intracellular vesicle water channel with anion permeability that is activated by low pH or HgCl2 (PMID: 12034750). It could be involved in physiological mechanisms of fluid movement, acid-base regulation, and/or anion transport. The calculated MW of AQP6 is 29 kDa, but the actual observed MW is around 35 kDa, which represents glycosylated AQP6 monomer forms (PMID: 19811639).