AQP7 Polyklonaler Antikörper

AQP7 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 25131-1-AP

Synonyme

AQP 7, AQP7, AQP7L, AQPap, Aquaglyceroporin 7, aquaporin 7, Aquaporin 7 like, Aquaporin adipose

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  WB analysis using 25131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25131-1-AP (AQP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 25131-1-AP

  IP result of anti-AQP7 (IP:25131-1-AP, 4ug; Detection:25131-1-AP 1:300) with mouse kidney tissue lysate 6500ug.

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  IHC staining of human kidney using 25131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25131-1-AP (AQP7 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25131-1-AP (AQP7 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 25131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25131-1-AP (AQP7 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 25131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25131-1-AP (AQP7 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maushodengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

25131-1-AP bindet in WB, IHC, IF, IP, ELISA AQP7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AQP7 fusion protein Ag17945
• Vollständiger Name: aquaporin 7
• Berechnetes Molekulargewicht: 342 aa, 37 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa, 40 kDa
• GenBank-Zugangsnummer: BC062701
• Gene symbol: AQP7
• Gene ID (NCBI): 364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AQP7 is a member of the aquaporin family of water-selective membrane channels, localizes to the plasma membrane, and allows movement of water, glycerol and urea across cell membranes. AQP7 forms a channel that mediates water and glycerol transport across cell membranes at neutral pH. AQP7 is highly expressed in the adipose tissue(PMID: 9405233).

Publikationen

Species	Application	Title
mouse	IF	Gastroenterology; Loss of MYO5B Leads to Reductions in Na+ Absorption With Maintenance of CFTR-Dependent Cl- Secretion in Enterocytes.; Authors - Amy C Engevik
mouse	IF	FASEB J; Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia; Authors - Sheng Gao
sheep	IF	Animals (Basel); Rumen-Degradable Starch Improves Rumen Fermentation, Function, and Growth Performance by Altering Bacteria and Its Metabolome in Sheep Fed Alfalfa Hay or Silage; Authors - Wenliang Guo
mouse	IHC	Biomed Pharmacother; Central administration of Dapagliflozin alleviates a hypothalamic neuroinflammatory signature and changing tubular lipid metabolism in type 2 diabetic nephropathy by upregulating MCPIP1; Authors - Jingjing Da