AQP9 Polyklonaler Antikörper

AQP9 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20380-1-AP

Synonyme

HsT17287, Aquaporin-9, Aquaglyceroporin-9, Aquaglyceroporin 9, AQP-9

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  WB analysis using 20380-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20380-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20380-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20380-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20380-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 20380-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 20380-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human liver using 20380-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver using 20380-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 20380-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Maushirngewebe, Mauslebergewebe, Mauslungengewebe, Maushodengewebe, Rattenhirngewebe, Rattenlebergewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Lebergewebe, Maushirngewebe, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

20380-1-AP bindet in WB, IHC, IF-P, CoIP, ELISA AQP9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AQP9 fusion protein Ag14200
• Vollständiger Name: aquaporin 9
• Berechnetes Molekulargewicht: 295 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC026258
• Gene symbol: AQP9
• Gene ID (NCBI): 366
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aquaporins (AQPs), also known as water channel proteins, are members of a large protein family that osmotically modulate water fluid homeostasis in several tissues. AQP9 (Aquaporin 9) is a member of the aquaporin family that acts as a water-selective membrane channel, and it may be involved in cell migration, angiogenesis, and tumor growth. AQP9 is involved in arsenic uptake in hepatocellular carcinoma cells, in which p38-MAPK may play a limited role. AQP9 was demonstrated to promote astrocytoma cell invasion and motility via the AKT pathway.

Publikationen

Species	Application	Title
mouse	WB,IHC	Int J Med Sci; Exploring the Shared Diagnostic Genes in IBD and Psoriasis through Bioinformatics and Experimental Assays; Authors - Lichun Han
human	WB,IHC,CoIP	Sci Rep; Aquaporin 9 downregulation in KRASG12V colorectal cancer and associated with increased proliferation and decreased apoptosis in cancer cells; Authors - Yankui Liu ; KD Validated