AQR Polyklonaler Antikörper

AQR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 24342-1-AP

Synonyme

KIAA0560, Intron-binding protein of 160 kDa, IBP160, fSAP164, EC:3.6.4.13

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  WB analysis of HEK-293 using 24342-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24342-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24342-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24342-1-AP

  IP result of anti-AQR (IP:24342-1-AP, 4ug; Detection:24342-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human kidney using 24342-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

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  IHC staining of human brain using 24342-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

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  IHC staining of human brain using 24342-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 24342-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

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  IF Staining of U2OS using 24342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AQR antibody (24342-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of U2OS using 24342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AQR antibody (24342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

24342-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA AQR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AQR fusion protein Ag17810
• Vollständiger Name: aquarius homolog (mouse)
• Berechnetes Molekulargewicht: 1485 aa, 171 kDa
• Beobachtetes Molekulargewicht: 160-171 kDa
• GenBank-Zugangsnummer: BC127111
• Gene symbol: AQR
• Gene ID (NCBI): 9716
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Mol Cell; Efficient Pre-mRNA Cleavage Prevents Replication-Stress-Associated Genome Instability.; Authors - Federico Teloni
human	WB,CoIP	Sci Adv; FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production; Authors - Weilong Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Did work perfectly to detect Aquarius by WB in HeLa extracts from HeLa cells. (1/1000 dilution, Overnight incubation at 4°C)

• Applications: Western Blot
• Cell Tissue Type: HeLa