androgen receptor Monoklonaler Antikörper

androgen receptor Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

CloneNo.

1F7C12

Kat-Nr. : 66747-1-Ig

Synonyme

androgen receptor,AR, AR, 1F7C12, DHTR, NR3C4

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  WB analysis using 66747-1-Ig

  PC-3(AR-), DU 145(AR-) and LNCaP (AR+) cell lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66747-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66747-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:8000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human prostate cancer using 66747-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human prostate cancer using 66747-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of LNCaP using 66747-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, humanes Hodengewebe, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Prostatakarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:600-1:3000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66747-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA androgen receptor und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: androgen receptor fusion protein Ag17291
• Vollständiger Name: androgen receptor
• Berechnetes Molekulargewicht: 914 aa, 99 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC132975
• Gene symbol: AR
• Gene ID (NCBI): 367
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AR, also named as DHTR and NR3C4, belongs to the nuclear hormone receptor family and NR3 subfamily. AR is a ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. AR is activated, but not phosphorylated, by HIPK3. Defects in AR are the cause of androgen insensitivity syndrome (AIS), previously known as testicular feminization syndrome (TFM), which is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) which also known as Kennedy disease. Defects in AR may play a role in metastatic prostate cancer. Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) which also known as Reifenstein syndrome. AR exists various isoforms with MW 110-120 kDa and 75-80 kDa. (PMID: 19244107 )

Publikationen

Species	Application	Title
Human	IF	Cell Stem Cell; Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.; Authors - Ryan M Samuel
rat	IHC	Development; Human prostate organoid generation and the identification of prostate development drivers using inductive rodent tissues; Authors - Parmveer Singh
rat	WB	Chin J Nat Med; Eucommia lignans alleviate the progression of diabetic nephropathy through mediating the AR/Nrf2/HO-1/AMPK axis in vivo and in vitro; Authors - Qi Huang
mouse,human	WB,IP,IF	iScience; WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation; Authors - Kai Song
human	WB,IF	Gynecol Endocrinol; N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway; Authors - Yuan-Xue Jing
rat	WB	Psychoneuroendocrinology; Effects of testosterone dose on depression-like behavior among castrated adult male rats; Authors - Zhongyu Ren