ARC Monoklonaler Antikörper

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• KD/KO Validated

ARC Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1G4B5

Rezensionen (1)

Kat-Nr. : 66550-1-Ig

Synonyme

ARC, ARC/ARG3.1, Arg3.1, KIAA0278

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Galerie der Validierungsdaten

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  WB analysis using 66550-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66550-1-Ig (ARC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66550-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66550-1-Ig (ARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66550-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66550-1-Ig (ARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 66550-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ARC antibody (66550-1-Ig, Clone: 1G4B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, Kaninchenhirngewebe, Maus-Cerebellum-Gewebe, Maushirngewebe, Neuro-2a-Zellen, Ratten-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

66550-1-Ig bindet in WB, IHC, IF, ELISA ARC und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARC fusion protein Ag9445
• Vollständiger Name: activity-regulated cytoskeleton-associated protein
• Berechnetes Molekulargewicht: 396 aa, 45 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC012321
• Gene symbol: ARC
• Gene ID (NCBI): 23237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC-Knockout HEK293 Cells.; Authors - Yu-Yuan Wang ; KD Validated
human,rat	WB,IF	Front Aging Neurosci; Methyltransferase-Like 3 Rescues the Amyloid-beta protein-Induced Reduction of Activity-Regulated Cytoskeleton Associated Protein Expression via YTHDF1-Dependent N6-Methyladenosine Modification.; Authors - Chenhaoyi Xu
mouse	WB	eNeuro; Phosphorylation of CREB at Serine 142 and 143 Is Essential for Visual Cortex Plasticity.; Authors - Nisha S Pulimood
rat	WB	Neurochem Res; Elevation of EGR1/zif268, a Neural Activity Marker, in the Auditory Cortex of Patients with Schizophrenia and its Animal Model.; Authors - Yuriko Iwakura
rat	WB	Neurotox Res; Inhibition of NLRP1-Dependent Pyroptosis Prevents Glycogen Synthase Kinase-3β Overactivation-Induced Hyperphosphorylated Tau in Rats; Authors - Xiangying Liu
rat	WB	Biomed Pharmacother; Protective effect of 3-n-butylphthalide against intrastriatal injection of malonic acid-induced neurotoxicity and biochemical alteration in rats; Authors - Chunling Yuan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pegah (Verified Customer) (08-24-2023)

I used this antibody 1:1000 for detection of Arc in whole brain lysates immunoprecipitated for Arc (with an anti-rabbit antibody).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000