ARCN1 Polyklonaler Antikörper

ARCN1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 23843-1-AP

Synonyme

Archain, archain 1, ARCN1, Coatomer subunit delta, COPD, Delta coat protein, Delta COP

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Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 23843-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23843-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 23843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23843-1-AP (ARCN1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 23843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23843-1-AP (ARCN1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 23843-1-AP

  Immunofluorescent analysis of HepG2 cells using 23843-1-AP (ARCN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

23843-1-AP bindet in WB, IHC, IF, ELISA ARCN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARCN1 fusion protein Ag20836
• Vollständiger Name: archain 1
• Berechnetes Molekulargewicht: 511 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa, 47 kDa
• GenBank-Zugangsnummer: BC093636
• Gene symbol: ARCN1
• Gene ID (NCBI): 372
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Archain 1 (ARCN1), also known as delta-COP, is a subunit of the coat protein I (COPI) complex, which comprises one of the protein coats that mediate vesicle budding from the membrane. Protein sequences of ARCN1 are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. The gene of ARCN1 has been identified in chromosome band 11q23.3, and sequencing of cDNAs suggests that at least two forms of mRNA with alternative 5' ends are present. (PMID: 7782067; 20502676)

Publikationen

Species	Application	Title
human	IHC	Nature; Cell stress in cortical organoids impairs molecular subtype specification.; Authors - Aparna Bhaduri
human	WB	Nat Chem Biol; Targeting colorectal cancer with small-molecule inhibitors of ALDH1B1.; Authors - Zhiping Feng
human	IF	bioRxiv; Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.; Authors - Madeline G Andrews
human	WB	Sci Adv; HPV is a cargo for the COPI sorting complex during virus entry; Authors - Mara C Harwood

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (05-14-2021)

very good for WB detection

• Applications: Western Blot

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